Small-scale purification of AAV produced with the AAV-MAX system

The Gibco™ AAV-MAX Helper-Free AAV Production System is an adeno-associated virus (AAV) production system developed for scalable high-titer AAV production. The AAV-MAX system comprises a clonal HEK293F-derived suspension cell line adapted to a chemically defined production medium, a transfection reagent and booster, a production enhancer, and lysis buffer. Here we present two strategies for bench-scale downstream processing and purification of recombinant AAV vectors produced using the AAV-MAX system. Both strategies include the use of the Thermo Scientific™ POROS™ AAVX affinity resin, with results demonstrating excellent recovery of AAV vectors.